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Pursuant to Section 20 C.F.R. 656.10, you are hereby notified that an application for Alien Employment Certification will be filed by Takeda Development Center Americas, Inc. for the following job opportunity:
JOB LOCATION: Cambridge, MA
POSITION : Research Senior Associate
POSITION DESCRIPTION : Takeda Development Center Americas, Inc. is seeking a Research Senior Associate with the following duties: design and execute in vitro antibody discovery campaigns using yeast and phage display techniques. Independently conduct complex experiments, and implement display technologies to discover and advance novel biotherapeutic products through discovery and optimization stages. Generate diverse repertoires from immunized animals to build immune libraries for discovery purposes. Design and build maturation libraries for display platforms (affinity/potency/ stability), leveraging datasets and software to improve lead candidates. Prepare samples for sequencing by NGS or Sanger methodologies, including data analysis. Perform mammalian cell culture, ELISA and flow cytometry experiments, including sorting by utilizing BD Canto, ARIA and HT systems. Develop and use molecular cloning strategies for antibody gene amplification, library construction, and for generating antibody diversities for display libraries. Complete molecular biology techniques including DNA and RNA extraction, PCR, RT-PCR, restriction enzyme digestions and site directed mutagenesis. Exercise technical judgement in execution of specialized procedures and interpretation of results. Initiate routine problem investigation and recommend solutions; perform calculations and basic analysis on experimental results and develop hypotheses. Contribute to design of well-defined experimental procedures; maintain responsibility for routine laboratory instrumentation and supplies; track, collect, organize and analyze data from matrix teams; required to track data from LIMS systems and maintain detailed electronic notebook. Up to 20% remote work allowed.
The estimated salary range reflects an anticipated range for this position. The actual base salary offered will be in accordance with state or local minimum wage requirements for the job location.
U.S. based employees may be eligible for short-term and/or long-term incentives. U.S. based employees may be eligible to participate in medical, dental, vision insurance, a 401(k) plan and company match, short-term and long-term disability coverage, basic life insurance, a tuition reimbursement program, paid volunteer time off, company holidays, and well-being benefits, among others. U.S. based employees are also eligible to receive, per calendar year, up to 80 hours of sick time, and new hires are eligible to accrue up to 120 hours of paid vacation.
REQUIREMENTS: Master’s degree in Biochemistry, Biotechnology, Molecular. Biology, Immunology Bioengineering, or related field, plus 1 year of related experience. Prior experience must include: design antibody discovery phage campaigns and strategize selections arms for given targets; build immune/single domain phage libraries using molecular biology techniques; independently handle and lead projects from discovery to clone selection for target discovery campaigns; design recombinant plasmids to generate overexpressing/knock-out cell-lines using molecular biology; develop high-throughput cell-based flow cytometry assays to screen potential antibody candidates to increase screening efficiency; laboratory experience in yeast and/or phage display, antibody discovery, molecular biology or related research role.
Full time. $86,500 - $148,300 per year.
Qualified applicants can apply at . Please reference job #R0175792 . EOE
Any individual may provide documentary evidence bearing on this application, with information on available workers and information on wages and working conditions, to the Certifying Officer, U.S. Department of Labor, Employment and Training Administration, Office of Foreign Labor Certification, 200 Constitution Avenue, NW, Room N-5311, Washington, DC 20210.
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